Biochem 101: How to design a Cannabis-equivalent citrus plant
Biochemically isolate all the required enzymes for the production of THC.
Perform N-terminal sequencing on isolated enzymes, design degenerate PCR (polymerase chain reaction) primers and amplify the genes.
Clone genes into an agrobacterial vector by introducing the desired piece of DNA into a plasmid containing a transfer or T-DNA. The mixture is transformed into Agrobacterium tumefaciens, a gram negative bacterium.
Use the Agrobacterium tumefaciens to infect citrus plants after wounding. The transfer DNA will proceed to host cells by a mechanism similar to conjugation. The DNA is randomly integrated into the host genome and will be inherited.