OK, we need some super hipster young college brainiacs looking for an adventure...imagine putting THC in Oranges so that you can get high drinking a glass of Orange Juice...it's been done. So, if it has been done with oranges, it only makes sense that it could be done with other plants, other fruits and vegetables...seems to me, that a THC organic salad would be just the way for our 20/30 somethings to start their evening, a great way for us Boomers to fend off our aches and pains. So, I would love to find some adventuresome people to do a bit of high tech DNA magic to create Tomatoes, Cucumbers and watermellon with THC...one of the reasons I am choosing these three specific fruits/vegetables, is they are a staple in juicing, a constant in almost every salad, and imagine how many seeds the first successful generation of seeds once the host plant has accepted the change into its genome, and passes the now inherited THC DNA down to each successive generation. What's the DEA and the FDA going to do when the THC found in Marijuana is now available in a HOST OF FRUITS AND VEGETABLES we eat on a daily basis!Biochem 101: How to design a Cannabis-equivalent citrus plant
Step One:
Biochemically isolate all the required enzymes for the production of THC.
Step Two:
Perform N-terminal sequencing on isolated enzymes, design degenerate PCR (polymerase chain reaction) primers and amplify the genes.
Step Three:
Clone genes into an agrobacterial vector by introducing the desired piece of DNA into a plasmid containing a transfer or T-DNA. The mixture is transformed into Agrobacterium tumefaciens, a gram negative bacterium.
Step Four:
Use the Agrobacterium tumefaciens to infect citrus plants after wounding. The transfer DNA will proceed to host cells by a mechanism similar to conjugation. The DNA is randomly integrated into the host genome and will be inherited.
